Journal: Antimicrobial Agents and Chemotherapy

Article Title: Vitamin C enhances corneal fungal infection treatment in mice via chemotaxis and anti-inflammation

doi: 10.1128/aac.01165-25

Figure Lengend Snippet: Modulated macrophage immune response and elevated neutrophil apoptosis induced by VC. Bone marrow-derived macrophages were co-cultured with spores for 3 hours, with or without VC, and the relative mRNA levels of IL-1β, TNF-α, IL-10, TGF-β, CASP-1, and HIF-1α were assessed by RT-PCR. Neutrophil apoptosis was evaluated via FACS analysis after 3 hours of VC or DHA exposure (bottom right). Mac, bone marrow-derived macrophage; N, neutrophils; DHA, dehydroascorbic acid, oxidized form of vitamin. P values are indicated as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significant difference.

Article Snippet: Bone marrow-derived macrophages were cultured from 8 to 12-week-old mice in macrophage medium ( ) (DMEM high-glucose medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin/streptomycin, 4 mM, and 20 ng/mL macrophage colony-stimulating factor [GM-CSF, #HY-P7069, MCE]) for 7 days.

Techniques: Derivative Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction